Peptide Purification

To address the dual needs for larger scale production and lower cost-of-goods produced for new therapeutic peptides, Dow has developed a full range of media for Reversed-Phased Chromatography (RPC) under the Amberchrom trademark.

AMBERCHROM™ chromatographic media have unique surface chemistries which offer distinctive selectivity for biomolecules. The effectiveness of these products has been demonstrated in commercial-scale production processes for therapeutic peptides for over a decade.

Due to its high resolving power, RPC is frequently used to purify peptides from either synthetic reaction mixtures or biological sources. Depending on the process requirements for throughput, capacity, and resolving power, an Amberchrom™ resin product can be used to help developers optimize their overall process.

A chemically robust chromatography resin can not only improve the cost of existing products, but can also enable the production of new products that might not otherwise be feasible to manufacture economically, as illustrated in the influenza peptide example.

References

Dahma, H., Gourlet, P., Vandermeers, A., Vandermeers-Piret, M-C., and Robberecht, P., Peptides, 22 (2001) 1491-1499.

Hsiung, H.M., Smiley, D.L., Zhang, X., Zhang, L., Yan, L.Z., Craft, L., Heiman, M.L., and Smith, D.P., Peptides, 26 (2005) 1988-1996.

Marshall, N.B., Oda, S.K., London, C.A., Moulton, H.M., Iversen, P.L., Kerkvliet, N.I., and Mourich, D.V., J. Immunol. Meth., 325 (2007) 114-126.

Related Information

The Effect of Mobile Phase Additives on the Analysis of Peptides and Proteins. (pdf file: 1400 kb)