March 26-28, 2008
Interphex 2008
Pennsylvania Convention Center, PA, USA
Meet our healthcare experts at booth B 4015
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May 18-21, 2008
TIDES 2008
Red Rock Resort - Las Vegas, NV
Meet our Advanced Biosciences experts at booth 416
Jon Fisher will be presenting a poster on:
Effect of Mobile Phase Conditions on the Purification of Vancomycin Peptide (pdf file)
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June 15-18, 2008
PREP 2008
San José, CA, USA
Tuesday June 17, 2008 from 3.50 pm – 4:10 pm
Quantitative Determination of the Effect of Column Packing on Dynamic Capacity Measurement
Paper presented by Jon Fisher
Monday June 18 2:10pm - 3:20pm
A New Cost-Effective Adsorbent for the Capture and Purification of Antibiotics (pdf file)
Poster presented by Jon Fisher
July 17-18, 2008
NEXT GENERATION VACCINES
Gaylord National Resort and Convention Center
National Harbor, MD
Poster presentation:
Abstract:
Detergents are commonly used in hydrophobic cell membrane protein purification, vaccine purification and viral removal/inactivation. These detergents must eventually be removed during the purification process, and there are a number of methods for detergent removal, including dialysis, size exclusion chromatography, and filtration. However, if the detergent's critical micellar concentration (CMC) is low some of these methods may be ineffective. We investigate the effectiveness of Amberlite™ polymeric adsorbents for the removal of several non-ionic and ionic detergents from protein solutions. By using a batch-wise approach we are able to efficiently remove detergent while sustaining high protein recoveries. This is an economical technique that can be easily employed at lab or production scale.
August 3-8, 2008
7th Joint Meeting of AFERP, ASP, GA, PSE & SIF
Natural Products with Pharmaceutical, Nutraceutical, Cosmetic and Agrochemical interest
Athenaeum Intercontinental, Athens, Greece
August 6, 2008
Abstract:
Polymeric adsorbents offer a more efficient, cost-effective, and environmentally-friendly way to recover natural products than traditional activated carbon resins. Unlike activated carbon resins, polymeric adsorbents are both regenerable and free from heavy metal contaminates. It is well known that polyphenolic compounds, which are responsible for bitterness or color in natural extracts, have become more and more interesting commercially. Herein we demonstrate the efficiency and utility of a new food grade adsorbent, AMBERLITE™ FPX66 in extracting polyphenolic compounds from natural product.
The first application to be studied was debitterring and decolourization of yeast extract with AMBERLITE™ FPX66. This adsorbent had a very high reduction factor in colour (twelve-fold) in the first run, and even retained a relatively high efficiency (four fold reduction) after 100 CV (Column volumes) had passed the column. The bitterness level was judged to have been significantly reduced as well.
The second application was the adsorption, debittering, and recovery of polyphenolic compounds from both pomegranate extract and cranberry juice (prime juice or waste part of plants). A high concentration was yielded from FPX66 with cranberry, while a high yield at purity greater than 50% was obtained from pomegranate. The resin was economically regenerated to its original white color, indicating the ease and suitability for clean in place protocols.
Adsorbent technology, based on a wide range of chemistries - polystyrenic, polyacrylic or formophenolic - offers a broad range of applications, such as neutraceuticals recovery for functional food, active pharmaceutical ingredient from plant, and natural hormones extraction.
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September 23-26, 2008
BioProcess International
Disneyland Hotel, Anaheim, CA
Poster presentation
Abstract:
One of the biggest challenges in biopharmaceutical production is the reduction of costs. Increased production titers in products such as monoclonal antibodies have now created bottlenecks in downstream processing. High titer (>60 OD) bacterial cultures can be very darkly colored, and this color can bind tightly to anion exchange media. For larger capture columns, the installed cost of a packed column can be hundreds of thousands of dollars. Therefore, it is important to ensure that the media lifetime is sufficient to provide good process economics. One strategy for improving chromatographic column lifetime is the use of inexpensive, large particle size anion exchange resins for the removal of column-fouling color bodies prior to further purification. Amberlite™ FPA resins are economical choices for upstream color and contaminant removal. These resins function essentially as a guard column for the more expensive resins, and reduce the need for more frequent column repacking and media replacement. In this study, we demonstrate protocols for effective decolorization of fermented product process streams. This includes protocols for both smaller molecules, such as vancomycin, and larger recombinant proteins. Results demonstrate that Amberlite™ FPA resins are effective for decolorizing these feed streams while providing excellent product recovery.
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September 28 - October 1, 2008
SPICA 2008
Symposium on Preparative & Industrial Chromatography and Allied Techniques, Zurich, Switzerland.
Meet our Advanced Biosciences experts at booth 4
Laurent Joron will be presenting 2 posters on:
Abstract:
Within the pharmaceutical and biopharmaceutical industry, there are many process streams which require purification by some means or another. Typically, this requires that a specific impurity is removed or that a range of impurities are eliminated. In the latter case, these impurities are never fully characterized and are generally given the name “color species”. In all cases, the “color” is regarded as an impurity which must be removed in order to avoid any adverse quality problems downstream with the final pharmaceutical product.
Color in a process stream can be derived from a variety of different sources like fermentation from which the product is derived or degradation products. The molecular weight of these degradation products vary greatly but tend to have a carboxylic functionality.
These “color species” can foul and discolor resins used in the upstream portion of purification processes. Fouling and discoloration of expensive ion exchange resins can be avoided by the use of inexpensive ion exchange resins that can be used econmically in a disposable mode. We demonstrate protocols for effective decolorization of fermented product process streams.
Abstract:
Detergents are commonly used in hydrophobic cell membrane protein purification and viral removal/inactivation. These detergents must eventually be removed during the purification process, and specifically in vaccines production.
A rapid detergent removal protocol using short chromatographic columns has been developed. A number of different polymeric resins were screened for detergent removal. A polystyrene divinylbenzene polymer, Amberchrom™ CG161, and an acrylic polymer, Amberchrom™ CG71C, were employed for the removal of non ionic detergents from protein solutions. The results demonstrate that these resins exhibited high capacity and were effective in removing a wide range of detergents at high linear velocities with minimal loss in protein yield.
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October 7-9, 2008
BIOTECHNICA 2008
Hannover, Germany
Meet our Advanced Biosciences experts at Stand G39. Hall 9
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October 20-22, 2008
Viral Vectors and Vaccines
13th International Meeting
Westin Galleria, Houston, TX
Poster presentation:
Abstract:
Detergents are commonly used in hydrophobic cell membrane protein purification and viral removal/inactivation. These detergents must eventually be removed during the purification process, and specifically in vaccines production.
A rapid detergent removal protocol using short chromatographic columns has been developed. A number of different polymeric resins were screened for detergent removal. A polystyrene divinylbenzene polymer, Amberchrom™ CG161, and an acrylic polymer, Amberchrom™ CG71C, were employed for the removal of non ionic detergents from protein solutions. The results demonstrate that these resins exhibited high capacity and were effective in removing a wide range of detergents at high linear velocities with minimal loss in protein yield.
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